Development of Novel Assays for Proteolytic Enzymes Using Rhodamine-Based Fluorogenic Substrates
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چکیده
منابع مشابه
Rhodamine-based compounds as fluorogenic substrates for serine proteinases.
A new fluorogenic substrate for serine proteinases, bis(N-benzyloxycarbonyl-L-argininamido)Rhodamine [(Cbz-Arg-NH)2-Rhodamine], was synthesized, purified and chemically and enzymically characterized. This compound, which employs Rhodamine as a fluorophoric leaving group, is the first in a series of substrates designed to measure the amidase activity of proteinases. Cleavage of one of the amide ...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Screening
سال: 2002
ISSN: 1087-0571,1552-454X
DOI: 10.1177/1087057102238627